In exchange for carbohydrate energy resources, the fungus provides benefits to the plant which can include increased water or nutrient uptake and. Fusarium is the dominant genus in synthetic media isolated from the root of dendrobium moniliforme. Pdf isolation and identification of fungal endophytes. Isolation, characterization of endophytic fungi of. Of the 330 isolates recovered, 212 were from roots, 97 from leaves and 21 isolates from stems with a prevalence of 3.
A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. Antimicrobial activity screening of endophytic fungi. Diversity estimation and antimicrobial activity of culturable. Aro a c dorcas gado a ajit kumar passari d vineet kumar mishra d bhim pratap singh d e lyndy j mcgaw a.
Colonization and isolation rates of endophytic fungi from medicinal plants. Antimicrobial activity of crude extracts of endophytic. This study aims to evaluate the antimicrobial activities of endophytic fungi from marine brown algae padina sp. Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Plant use of endophytic fungi in defense wikipedia. Pdf endophyte fungi reside in the tissues beneath the epidermal cell layers and live within a plant for at least part of its life without causing. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. The fungus colletotrichum falcatum was found to be the coregroup fungus with the colonization frequency of 12. Isolation of endophytic fungi from south african plants. Collection of samples and isolation of endophytic fungi.
Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. Isolation, purification and maintenance of the endophytic fungus producing vinblastine and vincristine. These include both culturable and unculturable bacteria belonging to acidobacteria, actinobacteria. The isolation of endophytic actinomycetes from surfacesterilized tissues of 36 plant species was made using humic acidvitamin hv agar as a selection medium. Endophytic fungi were isolated from endemic medicinal plants of tirumala hills by the procedure of standardized and modified method described by hallman et al. Isolation of endophytic fungi from conyza blinii h. Endophytic fungi residing in medicinal plants have not been systematically characterized. A total 11 endophytic fungi were isolated from two plants collected from amravati district of maharashtra region for obtaining biological diversity in endophytic fungi.
The fossil record indicates that plants have been associated with endophytic krings et al. Isolation and identification of endophytic fungi from 10 species palm trees. Svacica 1d, 3 osijek, croatia 2agricultural institute osijek, juzno predgrade 17, 3 osijek, croatia. Isolation and identification of lasparaginaseproducing. Redlin sc, carris lm eds endophytic fungi in grasses and woody plants. Endophytic fungi were isolated from the bacopa monnieri. Pdf isolation and screening of endophytic fungi from three plants. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2 1department of plant science and technology, huazhong agricultural university, wuhan, hubei, p. Results and discussion total of 14 endophytic fungi were isolated from 610 samples of both leaves and stem of different parts of endemic plants table 1. Previous studies have reported the isolation and identification of endophytic fungi and bacteria from medicinal plants such as cressa cretica, achillea. A total of 153 endophytic fungi were isolated from 94 fresh plants and purified from 1974 tissue segments stems, leaves, and roots of. Pdf isolation and characterization of endophytic fungi. A total of nine morphotypes out of 50 isolated strains were recovered in the isolation procedure from root pieces table 1. Isolation and identification of endophytic fungi from 10.
Endophytic fungi are those living inside the host plant without causing any apparent negative effect on host plant. An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease. The plants were collected from 4 dif erent localities which includes seethampundi tamil nadu, yediyur karnataka, allepy kerala and udupi karnataka. This study was conducted to isolate lasparaginaseproducing endophytic fungi from medicinal plants of family asteraceae. China 2department of plant sciences, faculty of biological sciences, quaidiazam. Endophytic bacteria with plant growth promotion and. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Mundt and hinkle, 1976 more than 200 bacterial genera from 16 phyla have been reported as endophytes. Bills gf 1996 isolation and analysis of endophytic fungal communities from woody plants. The healthy plants without disease symptoms or pest infestation were selected. Lasparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. A friendly relationship between endophytic fungi and. Plant samples were collected from 2 varieties of rice, khao jow hawm suphan buri and pathum thani 80. Isolation and screening of extracellular enzymatic.
Studies autonomous, lawspet, pondicherry605008, india abstract. A total of six 6 endophytic fungi were isolated from 20 samples of a. The samples were rinsed gently in running water to remove dust and debris. Among them, endophytic bacteria are thought to interact closely with their host plants, and therefore could be used as biological control agents in sustainable crop production potentially sturz and nowak, 2000. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. Research article diversity of endophytic fungi from first.
Rosenblueth and martinez 2006 which could mean that endophytes have a role in metabolizing these substances. Fiftyfour endophytic fungi with different morphologies were isolated from the leaves, stems and roots of tribulus terrestris l. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity. Isolation and identification of endophytic fungi from srikaya plants annona squamosa having potential secondary metabolites as antibreast cancer activity. The present study is aimed at the isolation and identification of endophytic fungi from p. The percentage occurrence of endophytic fungi varies with the host plants. First, the tissue masses were immersed in 75% ethanol solution for 1 min, followed by 4% sodium hypochlorite solution for 3 min. These isolated inhibit growing of pathogenic bacteria s. Isolation of endophytic fungi was done according to the method described by petrini 1986. Biodiversity of phylloplane and endophytic fungi have been considered as one of the rich. Isolation and identification of a novel endophyte from a. Since the first reliable reports about the isolation of endophytic bacteria from surfacesterilized plants samish et al. In this study, we classified 1160 fungal isolates from 29 medicinal plant species using traditional.
Isolation and screening of endophytic fungi from three plants used in traditional medicine in nigeria for antimicrobial activity. Isolation procedures for endophytic microorganisms. Identification of endophytic fungi from fruits and. Nine endophytic fungi were isolated from the root sections and characterized by molecular technique. In this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. A total of 25 morphologically distinct endophytic fungi were isolated. Hence, it is of utmost importance to study the kind of endophytes harbored by these plants. Relative abundance of endophytic fungi after isolation in synthetic media. Two endophytic fungi were isolated each from the root parts, leaf parts and stem parts. Isolation and identification of endophytic fungi from medicinal plants and assessment of their antimicrobial activity. Endophytic fungi isolated from four different populations of urginea indica are reported in the present report. Figure 2 preparation ofsurface sterilization agarwood and isolation of 8 endophytic fungi from agarwood. After molecular analysis, all morphotypes looked homogeneous in terms of internal transcribed spacer its sequence and all belonged to ascomycota table 1, in the genera cladosporium. The aim of this study was to identify the endophytic fungi from medicinal plant ricinus communis and to investigate their potential antibacterial activity.
This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Isolation of soil and endophytic fungi from rice oryza. The ability of endophytic fungi of grasses to provide protection from insect herbivores has drawn the attention to study the endophytic microflora especially fungi for better health of crop plants and their bioactivity against wide range of plant and pathogens, there has been increasing interest in the investigation of endophytic fungi. Pdf isolation and identification of endophytic fungi. Endophytic bacteria reside within plant hosts without causing disease symptoms. Isolation and identification of phylloplane and endophytic fungi from one ornamental plant,mangifera indica bijaya kumar nayak department of botany, k. The present study aims to identify the diverse endophytic fungi residing in the roots of dendrobium moniliforme and their role in plant growth and development. We determined several phenotypic properties and found approximately equal numbers of gramnegative and grampositive isolates. The collected plants were identified according to the morphological charaters shown as the literatures of backer 1968, hooker 1885 and lawrence 1951. Isolation and characterization of endophytic colonizing. Antimicrobial activity and phytochemical analysis of crude. Cytotoxic and antimicrobial activities of endophytic fungi.
Five different taxa were represented by 11 isolates, and six isolates. Isolation and identification of endophytic fungi from. Screening, isolation, biochemical and plant growth. Endophytes are ubiquitous and have been found in all species of plants studied to date. Isolation and characterization of endophytic fungi from. The endophytes were isolated using a modified method described by arnold et al. It is an important medicinal plant found distributed in india, africa and mediterrean regions and widely used for. Quantification of the indole acetic acid iaa compound by these endophytes was done. Isolation and identification of flavonoidproducing. Endophytes are endosymbionts and often bacteria, fungi and actinomycetes that live asymptomatically within plants.
Influence of endophytic fungi isolated from symptomless. The material was thoroughly washed in sterile water, surfacedisinfected by soaking in 70% ethanol for 30 sec and 0. The plants samples were rinsed gently in running water to remove dust and debris. Unlike mycorrhizal fungi that colonize plant roots and. Isolation of endophytic actinomycetes from selected plants. Isolation of endophytic fungi and screening of huperzine a. Abstract endophytic fungi isolated from four different populations of urginea indica are reported in the present report. After molecular analysis, all morphotypes looked homogeneous in terms of internal transcribed spacer its sequence and all belonged to ascomycota table 1, in the genera cladosporium, colletotrichum, cylindrocarpon. The plant material was subsequently rinsed in sterile demineralised water. Plant use of endophytic fungi in defense occurs when endophytic fungi, which live symbiotically with the majority of plants by entering their cells, often fungus or bacteruim, are utilized as an indirect defense against herbivores. Endophytic fungi or endophytes exist widely inside the healthy tissues of living plants, and are important components of plant microecosystems.
Isolation of endophytic fungi isolation of endophytic fungi was done according to. Endophytic fungi from medicinal plants wiyakrutta et al. Influence of endophytic fungi isolated from symptomless weeds on cherry plants ilic j1, cosic j1, vrandecic k1, dugalic k2, pranjic a2 and martin j3 1faculty of agriculture in osijek, k. Isolation of endophytic fungi from south african plants, and screening for their antimicrobial and extracellular enzymatic activities and presence of type i polyketide synthases author links open overlay panel muna ali abdalla a b abimbola o. The leaves and stems were washed with running water and immersed in distilled water until the samples were saturated and restored.
Furthermore biodiversity of endophytic fungi in various segments of the plant were determined by statistical analysis simpson index 1d. Endophytic fungi live within healthy plant parts such as leaves, stems and roots arnold, 2007 and are an important source of effective bioactive compounds. Isolation and identification of phylloplane and endophytic. Isolation of endophytic fungi from thermopsis lanceolata and their antioxidant activity 29 name of genus fungi number root stem leaf alternaria 5 3 2 aspergillus 2 2 0 chaetomium 1 0 0 fusarium 1 0 0 totalpercentage 956. Over the long period of evolution, some coexisting endophytes and their host plants have established a special relationship with one and another, which can significantly influence the formation of metabolic products in plants, then. Endophytic fungi that are residing asymptomatically in internal tissues of all higher plants are of growing interest as promising sources of biologically active agents. Isolation and characterization of plant growthpromoting. Cytotoxic and antimicrobial activities of endophytic fungi isolated from bacopa monnieri l. Endophytic fungi are symbiotically associated with plants and can synthesize the same bioactive compounds and natural products as their host plant themselves, suggesting the possibility of intergeneric genetic exchange between the plant and the fungus.
9 187 1060 1119 1382 300 1414 1040 1323 131 147 526 178 1087 1140 143 717 909 1476 1293 123 744 380 1485 905 1482 32 447 1379 833 256 1303 61